Professor Nakagata retired at Division of Reproductive Engineering and launched a next Project.4/14/2020 March 31, 2020: Professor Naomi Nakagata completed his 22-years career at Division of Reproductive Engineering in Center for Animal Resources and Development (CARD), Kumamoto University in Japan. He developed an excellent technique of mouse sperm cryopreservation called as "Nakagata Method". Using the technique, he developed CARD Mouse Bank System to efficiently archive and distribute genetically engineered mice. In addition, he organised hand-on workshops of mouse reproductive technology in Japan, Korea, China, Taiwan, Singapore, Spain, France and USA. His achievements significantly impacted on the filed of mouse genetics. Recently, he published a paper about rat mouse sperm cryopreservation in Scientific Reports. He will continue his new project at Division of Reproductive Biotechnology and Innovation at CARD in Kumamoto University. His new journey is started to discover key to solving problems in reproductive technology.
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Symposium on genetically modified mouse in biomedical science: Reproductive technology and usefulness as a disease model was held on July 5, 2019 at Khon Kaen University in Thailand. There were four lectures: Dr. Takeo, the benefits of reproductive technology in biomedical science, Prof. Okada (Center for Human Retrovirus Infection, Kumamoto University, Japan), application of highly immunocompromised mice for human cancer research, Associate Prof. Jirawatnotai (Mahidol University, Thailand), an acquired vulnerability of CDK4/6 Inhibitor-Resistant cholangiocarcinoma with therapeutic potential, and Dr. Vaeteewoottacharn (Khon Kaen University, Thailand), Patient-derived xenograft model for drug discovery. It was a fruitful symposium to share the latest information about the applications of reproductive technology and disease-model mouse in biomedical science.
Professor Naomi Nakagata visited Department of Laboratory Animal Science at Shanghai Jiao Tong University in Shanghai, China. There was a ceremony of the academic exchange agreement between Department of Laboratory Animal Science at Shanghai Jiao Tong University and Center for Animal Resources and Development (CARD) at Kumamoto University.
The 15th Transgenic Technology Meeting (TT2019) was held at Kobe International Conference Center in Kobe, Japan from April 7 to 10, 2019. The TT2019 Meeting was organized by Dr. Yasuhide Furuta (RIKEN BDR). In the meeting, Dr. Lluis Montoliu provided a lecture about transgenesis with large constructsas as the ISTT Prize Awardee.
After the meeting, the TT2019 workshop was held at RIKEN Center for Biosystems Dynamics Research (BDR) from April 11 to 13. Dr. Hiroshi Kiyonari organized the workshop. Fifteen participants attended to the workshop. The workshop included mouse reproductive technology (Dr. Takeo, CARD, Kumamoto University), Zygote electroporation, including GONAD (Dr. Ohtsuka, Tokai University), Manipulation of ova (pre- and post-fertilization) (Dr. Kyogoku, Dr. Kiyonari, RIKEN BDR), Microinjection of Xenopus embryos (Dr. Inomata, RIKEN), and Microinjection of zebrafish embryos (Dr. Phng, RIKEN). In the hand-on workshop of mouse reproductive technology, Dr. Jorge Sztein (CARD, Kumamoto University), Shuuji Tsuchiyam (CARD, Kumamoto University), Ayumi Mukunoki (CARD, Kumamoto University), and Satohiro Nakao (CARD, Kumamoto University) supported the workshop as instructor. It was a fruitful opportunity to share the latest techniques and knowledge of embryo manipulation in mammalian and non-mammalian species. We would like to thank to all participants, staff, sponsors, and ISTT committee. TT2019 Workshop in RIKEN BDR Asian Mouse Mutagenesis & Resource Association (AMMRA) and Asian Mouse Phenotyping Consortium (AMPC) Meeting was held at Walter and Eliza Hall Institute (WEHI) of Medical Research in Melbourne, Australia from Feb 20 to Feb 21, 2019. The 2019 AMMRA & AMPC meeting was organized by Dr. Michael Dobbie (AMMRA President and CEO, The Australian Phenomics Network) and Dr. Jim Hennessy (COO, The Australian Phenomics Network). The region’s leading clinicians, biomedical scientists, facility directors and senior executives were attended to the meeting from Australia (Australian National University, University of Queensland, Monash University, and WEHI), China (MARC, Nanjing University, and Institute of Biophysics, Chinese Academy of Sciences), Korea (Seoul National University DGIST, and Yonsei University), Taipei (National Laboratory Animal Center, Taiwan Mouse Clinic, and Institute of Molecular Biology), Japan (Riken BRC, and CARD, Kumamoto University), Canada (The Centre for Phenogenomics), and UK (MRC Harwell). The topics of the meeting was new research innovations and technological developments, future research requirements, and strategies to ensure that Asian capabilities for mouse production and phenotyping continue to lead the world. Dr. Toru Takeo (CARD, Kumamoto University) delivered progress report of CARD and a lecture about the latest reproductive technology in mice.
Program of the 2019 AMMRA & AMPC Meeting The 8th Congress of Asian Federation of Laboratory Animal Associations was held in Bangalore, India12/4/2018 The 8th Congress of Asian Federation of Laboratory Animal Associations (AFLAS) was held from November 29 to December 1 in Bangalore, India. The aim of AFLAS is to promotes through international co-operation Asian Congresses on Laboratory Animal Science for the purpose of reviewing scientific, technical and educational problems in Laboratory Animal Science, to develop other relevant activities in the interest of Laboratory Animal Science, and to contribute to animal welfare. Dr. Toru Takeo delivered a lecture about "Advances in Reproductive Technology" in the meeting.
AFLAS website: http://www.aflas-office.org/index.html The CARD-JAX Mouse Reproductive Technology Workshop was held at Jackson Laboratory in Bar Harbor, Maine (USA) on Oct 15 to 19, 2018. The workshop was organized by Naomi Nakagata and Toru Takeo of the Center for Animal Resources and Development (CARD) at Kumamoto University and Rob Taft, Jennifer Corrigan and Leanne Miceli of The Jackson Laboratory. Fifteen students participated in this 5 day event. Six lectures were provided by Dr. Larry Mobraaten (History of Mouse Bank), Dr. Jorge Sztein (History of Cryobiology), Prof. Naomi Nakagata (History of CARD), Dr. Toru Takeo (Two lectures of Ultrasuperovulation and Shipment of live embryos and sperm), and Dr. Barbara Stone (Non-surgical Embryo Transfer: NSET). Hands-on techniques covered embryo handling, oocyte, embryo and sperm cryopreservation and recovery, cold storage of embryo and sperm, surgical and non‐surgical embryo transfer, In‐vitro fertilization, flushing embryos, vasectomy, superovulation and inducing pseudopregnancy. Aseptic surgical techniques, biomethods and project planning were also be covered. It was a fruitful workshop to share the knowledge, experience and technology of mouse reproductive technology. We would like to thank to all participants, lecturers, instructors, JAX staff, shipment support (Chemical Dojin Co Ltd), travel grant support (Japan Agency for Medical Research and Development: AMED), and sponsors (KYUDO Co Ltd, Cosmo Bio Co Ltd, Howard Hughes Medical Institute: HHMI and Stoelting).
Website: CARD-JAX Mouse Reproductive Technology Workshop: https://cardjacksonmouse2018.weebly.com/ Dr. Toru Takeo and my team visited Jackson Laboratory (JAX) in Bar Harbor, Maine. It is fantastic environment with excellent training facility, instrument, accommodation and beautiful nature. Now, we are ready to host CARD-JAX Mouse Reproductive Technology Workshop in October 15 to 19, 2018. I'm very excited to have the unique collaborative workshop at JAX. Thank you, Rob, Jennifer, Charles and all JAX staff!
Our paper of cold-storage technique for sperm was selected as the BoR Most Popular Research Paper7/19/2018 Cold-transport technique of sperm is very useful to transport genetic resource of mammals. Last year, we published a paper about novel compounds of dimethyl sulfoxide and quercetin to prolong the viability and fertility of cold-stored sperm in the scientific journal of Biology of Reproduction (BoR). In the annual meeting of the Society for the Study of Reproduction held in New Oreans, our paper was selected as the Most Popular Research Paper in 2017. Dr. Hidetaka Yoshimoto attended the ceremony and received the award as representative of our team in the conference.
It is great honor to invite Dr. Zhang Qin (Institute of Biophysics, Chinese Academy of Sciences), Zhang Jing (Laboratory Animal Research Center of Tsinghua University) and Li Yang (Laboratory Animal Research Center of Tsinghua University) to CARD, Kumamoto University. We shared information about the management of mouse bank system, reproductive technology and the latest data of our research. At the 218th CARD seminar, Li Yang introduced Laboratory Animal Research Center of Tsinghua University, Zhang Jing talked about the management of Mouse IVF and microinjection group and Dr. Zhang Qin lectured unique interaction between bacterial ligands and vesicle trafficking system. It was a fruitful collaboration to improve research infrastructure and establish a robust global network of research institutes for growing life science community.
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April 2020
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